ABSTRACT
Objective:To explore the relevance of the three polymorphic loci in the CUB and SUSHI multiple domains 1(CSMD1) gene with schizophrenia and its cognitive function.Methods:Polymerase chain reaction-restrictive fragment length polymorphism (PCR-RFLP) was used to detect CSMD1 gene polymorphism in 109 schizophrenia patients(case group) and 109 healthy controls(control group), and the cognitive function was evaluated by Montreal cognitive assessment (MoCA). The allele and genotype frequencies between the case group and the control group were statistically analyzed by SPSS 19.0 software.Results:(1)There were significant differences in the distribution of genotype AA, AC, CC (case group: AA, 51(46.79%), AC, 43(39.45%), CC, 15(13.76%); control group: AA, 22(20.18%), AC, 58(53.21%), CC, 29(26.61%), χ2 =18.203, P<0.001) and allele frequencies (case group: A, 145 (66.51%), C, 73 (33.49%); control group: A, 102(46.79%), C, 116(53.21%), χ2=17.269, P<0.001, OR=0.443, 95% CI: 0.301-0.652) at rs10503253 loci between the case group and the control group.(2) Differences in allele frequencies at the rs10503253 loci were associated with the visual space and executive dysfunction in the case group ( χ2=6.470, P=0.011, OR=2.089, 95% CI: 1.179-3.702). (3) Differences in allele frequencies at the rs17405197 loci were associated with language dysfunction in the case group ( χ2=9.468, P=0.002, OR=0.415, 95% CI: 0.235-0.731). (4) Differences in allele frequencies at the rs2740931 loci were associated with language dysfunction in the case group ( χ2=5.094, P=0.024, OR=2.016, 95% CI: 1.091-3.726). Conclusion:CSMD1 gene polymorphism may be a risk factor for the onset of schizophrenia, associated with symptoms of cognitive dysfunction in schizophrenia.
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Objective:To analyze the research status of gouty arthritis at home and abroad in the past 15 years，and to provide reference for for further in-depth research in this field. Method:With “Gouty arthritis” as the research topic, the related papers during 2004—2019 were searched in Web of science core collection database and CNKI databases respectively. The results were statistically sorted out according to publication year，author，institution，literature source and key words，etc，and were visualized by CiteSpace software. Result:A total of 5 071 Chinese papers and 1 136 English papers were included. The amount of domestic and foreign publications continued to rise，forming a core team represented by LI Zhao-fu，XIONG Hui，SCHLESINGER NAOMI and other authors. The research hotpots focused on the pathogenesis，diagnosis methods， Chinese and Western medicine treatment，clinical observation，risk assessment and other aspects of gouty arthritis. Both of them had their own emphases. Domestic researches tended to focus on the treatment of gouty arthritis with traditional Chinese medicine，while foreign ones focused on the pathological research and clinical investigation of gouty arthritis. Conclusion:The number of researches in the field of gouty arthritis is on the rise as a whole，and there are both consistency and differences in the research content and hot topics between domestic and foreign literature. Therefore， we should strengthen cooperation and exchange between different teams and countries，so as to promote the overall development of this field.
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Objective To investigate the expression of miR-29s in the glioma stem cells,and explore how the members of miR-29s affect the bio-logical behaviors of glioma stem cells. Methods Eight patient specimens were used to culture glioma stem cells. Real-time PCR was adopted to test the expression of miR-29s. CCK-8 analysis was performed to test the proliferation ,Transwell was used to test cell migration and invasion ,and flow-cytometry analysis was carried out to test apoptosis. Results miR-29a,miR-29b and miR-29c were decreased in glioma stem cells. Over-ex-pression of miR-29s could inhibit the proliferation,cell migration and invasion,but promote apoptosis of glioma stem cells. Conclusion miR-29s acts as a cancer suppressor gene in the glioma stem cells ,and miR-29a plays the dominant functional role in the family.